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Recombinant Mouse VSIG4 cell lysate

Cat.No. : VSIG4-2519MCL
Product Overview : Mouse VSIG4 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Vsig4 V-set and immunoglobulin domain containing 4 [ Mus musculus ]
Official Symbol : VSIG4
Synonyms : VSIG4; V-set and immunoglobulin domain containing 4; V-set and immunoglobulin domain-containing protein 4; complement receptor of the immunoglobulin superfamily; CRIg; Z39IG; BC025105; A530061A11; MGC36211;
Gene ID : 278180
mRNA Refseq : NM_177789
Protein Refseq : NP_808457

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the relationship of VSIG4 to other genes or proteins? 08/18/2022

VSIG4 has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.

How can the disease be treated by regulating the levels of VSIG4? 05/09/2022

There are no drugs that directly regulate VSIG4 levels, but they can affect VSIG4 expression and function by modulating the immune system.

How to detect and analyze mutations in VSIG4? 02/08/2022

Mutations in VSIG4 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What is the specific role of VSIG4 in tumor immunity? 12/17/2020

VSIG4 plays an important role in tumor immunity, which can inhibit the activation and function of T cells and NK cells, thereby promoting tumor growth and metastasis.

How to study the regulatory mechanism of VSIG4? 06/05/2020

Studying the regulatory mechanism of VSIG4 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

What are the mutation types of VSIG4? 05/08/2020

The types of mutations in VSIG4 include point mutations, insertions/deletions, duplications, etc., which can cause structural and functional abnormalities of the protein.

Customer Reviews (3)

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Reviews
11/08/2021

    Stable performance, excellent performance in a variety of experiments.

    10/01/2021

      No side effects, high safety.

      03/13/2021

        It has good antigenicity and can induce target immune response.

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