Recombinant Human SERPING1 cell lysate
Cat.No. : | SERPING1-2615HCL |
Product Overview : | Human SERPING1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 [ Homo sapiens ] |
Official Symbol : | SERPING1 |
Synonyms : | SERPING1; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1; C1NH, serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary); plasma protease C1 inhibitor; angioedema; hereditary; C1 INH; C1IN; HAE1; HAE2; serpin G1; C1-inhibiting factor; C1 esterase inhibitor; complement component 1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1; C1NH; C1INH; |
Gene ID : | 710 |
mRNA Refseq : | NM_000062 |
Protein Refseq : | NP_000053 |
MIM : | 606860 |
UniProt ID : | P05155 |
Chromosome Location : | 11q12-q13.1 |
Pathway : | Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem; Pertussis, organism-specific biosystem; |
Function : | complement binding; peptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity; |
Products Types
◆ Recombinant Protein | ||
Serping1-729M | Recombinant Mouse Serping1 Protein, MYC/DDK-tagged | +Inquiry |
SERPING1-5004R | Recombinant Rat SERPING1 Protein, His (Fc)-Avi-tagged | +Inquiry |
SERPING1-999H | Recombinant Human SERPING1 Protein, MYC/DDK-tagged | +Inquiry |
Serping1-534R | Recombinant Rat Serping1 Protein, His-tagged | +Inquiry |
SERPING1-8058M | Recombinant Mouse SERPING1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
SERPING1-97H | Active Native Human C1 Esterase Inhibitor (C1-INH) | +Inquiry |
SERPING1-73H | Native Human C1 Esterase inhibitor | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionSERPING1 protein regulates the activity of C1 esterase inhibitor, which in turn modulates the activity of the complement system, leading to the characteristic symptoms of HAE.
Genetic mutations in the SERPING1 gene can lead to decreased levels or dysfunctional SERPING1 protein, contributing to the development of HAE.
Yes, monitoring SERPING1 protein levels can help assess the effectiveness of HAE treatments and guide adjustments in therapy if needed.
Yes, abnormal levels of SERPING1 protein have been implicated in conditions such as preeclampsia, ischemia-reperfusion injury, and sepsis.
Biotechnological advances are enabling the development of novel therapeutic modalities targeting SERPING1 protein, such as gene therapies and monoclonal antibodies.
Customer Reviews (3)
Write a reviewWith their expertise, I can navigate through experimental hurdles, optimize protocols, and ensure the seamless integration of the SERPING1 protein into my investigations.
The SERPING1 protein showcases unparalleled quality, ensuring it surpasses expectations and satisfactorily addresses my experimental requirements.
Their knowledgeable and responsive team stands ready to address any concerns or challenges I may encounter.
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