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Recombinant Human SERPING1 cell lysate

Cat.No. : SERPING1-2615HCL
Product Overview : Human SERPING1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 [ Homo sapiens ]
Official Symbol : SERPING1
Synonyms : SERPING1; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1; C1NH, serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary); plasma protease C1 inhibitor; angioedema; hereditary; C1 INH; C1IN; HAE1; HAE2; serpin G1; C1-inhibiting factor; C1 esterase inhibitor; complement component 1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1; C1NH; C1INH;
Gene ID : 710
mRNA Refseq : NM_000062
Protein Refseq : NP_000053
MIM : 606860
UniProt ID : P05155
Chromosome Location : 11q12-q13.1
Pathway : Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Hemostasis, organism-specific biosystem; Intrinsic Pathway, organism-specific biosystem; Pertussis, organism-specific biosystem;
Function : complement binding; peptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does the function of SERPING1 protein impact the pathophysiology of HAE? 04/18/2023

SERPING1 protein regulates the activity of C1 esterase inhibitor, which in turn modulates the activity of the complement system, leading to the characteristic symptoms of HAE.

What are the implications of genetic mutations in the SERPING1 gene for HAE patients? 12/08/2022

Genetic mutations in the SERPING1 gene can lead to decreased levels or dysfunctional SERPING1 protein, contributing to the development of HAE.

Can the measurement of SERPING1 protein levels be used to monitor treatment response in HAE patients? 04/25/2021

Yes, monitoring SERPING1 protein levels can help assess the effectiveness of HAE treatments and guide adjustments in therapy if needed.

Are there any other clinical conditions associated with aberrations in SERPING1 protein levels? 03/04/2021

Yes, abnormal levels of SERPING1 protein have been implicated in conditions such as preeclampsia, ischemia-reperfusion injury, and sepsis.

How is the role of SERPING1 protein in HAE treatment evolving with advances in biotechnology? 03/28/2019

Biotechnological advances are enabling the development of novel therapeutic modalities targeting SERPING1 protein, such as gene therapies and monoclonal antibodies.

Customer Reviews (3)

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Reviews
04/22/2023

    With their expertise, I can navigate through experimental hurdles, optimize protocols, and ensure the seamless integration of the SERPING1 protein into my investigations.

    05/14/2020

      The SERPING1 protein showcases unparalleled quality, ensuring it surpasses expectations and satisfactorily addresses my experimental requirements.

      12/02/2019

        Their knowledgeable and responsive team stands ready to address any concerns or challenges I may encounter.

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