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Recombinant Human ICAM1 cell lysate

Cat.No. : ICAM1-2655HCL
Product Overview : Human ICAM-1 / CD54 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : ICAM1 intercellular adhesion molecule 1 [ Homo sapiens ]
Official Symbol : ICAM1
Synonyms : ICAM1; intercellular adhesion molecule 1; BB2; CD54; human rhinovirus receptor; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; P3.58;
Gene ID : 3383
mRNA Refseq : NM_000201
Protein Refseq : NP_000192
MIM : 147840
UniProt ID : P05362
Chromosome Location : 19p13.3-p13.2
Pathway : Adaptive Immune System, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem;
Function : cell surface binding; integrin binding; protein binding; receptor activity; transmembrane signaling receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can ICAM1 be a potential therapeutic target? 07/11/2021

Yes, targeting ICAM1 is being explored as a potential therapeutic strategy for inflammatory diseases, with research focusing on developing ICAM1 inhibitors.

How is ICAM1 involved in inflammation? 07/09/2020

ICAM1 is upregulated during inflammation, promoting the adhesion of immune cells to the endothelium and facilitating their migration into inflamed tissues.

How is ICAM1 involved in cancer progression? 07/07/2019

ICAM1 expression can be altered in cancer cells and is implicated in promoting cancer progression by facilitating tumor cell adhesion, invasion, and metastasis.

Are there diagnostic applications for ICAM1 in clinical settings? 01/13/2017

ICAM1 levels can be measured in blood or tissue samples, and elevated levels may serve as diagnostic markers for certain inflammatory diseases.

Can ICAM1 be used as a prognostic marker in certain diseases? 08/13/2016

Elevated levels of ICAM1 in some diseases may serve as prognostic markers, indicating disease severity and potential outcomes.

Customer Reviews (3)

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Reviews
04/27/2022

    Manufacturers may provide documentation of the protein's validation, which includes information about its purity, concentration, stability, and potential contaminants.

    11/05/2020

      This can be particularly important in clinical trials to ensure accurate and reliable results.

      10/14/2016

        If the need arises for larger quantities of ICAM1 protein during the later stages of clinical trials, the manufacturer can facilitate scale-up production processes to meet those requirements.

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