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Recombinant Human CD200 cell lysate

Cat.No. : CD200-2707HCL
Product Overview : Human CD200 / OX-2 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CD200 CD200 molecule [ Homo sapiens ]
Official Symbol : CD200
Synonyms : CD200; CD200 molecule; antigen identified by monoclonal MRC OX 2 , CD200 antigen , MOX1, MOX2; OX-2 membrane glycoprotein; MRC; OX 2; CD200 antigen; MRC OX-2 antigen; antigen identified by monoclonal MRC OX-2; MOX1; MOX2; OX-2;
Gene ID : 4345
mRNA Refseq : NM_001004196
Protein Refseq : NP_001004196
MIM : 155970
UniProt ID : P41217
Chromosome Location : 3
Pathway : Adaptive Immune System, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem;
Function : identical protein binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does CD200 contribute to the regulation of bone marrow and hematopoietic stem cell niches? 10/04/2022

CD200 regulates bone marrow and hematopoietic environments, supporting stem cell maintenance and differentiation.

How does CD200 influence tumor immune evasion and the tumor microenvironment in cancer? 07/10/2021

CD200 contributes to immune evasion in cancers, affecting tumor progression and response to immunotherapy.

What role does CD200 play in the suppression of inflammatory responses in autoimmune diseases? 04/06/2021

CD200 plays a role in suppressing inflammatory responses, which is crucial in autoimmune disease management.

What are the effects of CD200 dysregulation on tissue graft rejection and transplant tolerance? 02/09/2019

Dysregulation of CD200 affects tissue graft rejection, influencing transplant tolerance and success.

How do alterations in CD200 expression impact the aging process and age-related immune changes? 09/17/2018

Changes in CD200 expression impact the aging immune system, affecting age-related immune regulation and function.

What is the impact of CD200 expression on neuroinflammatory processes and neurodegenerative diseases? 03/20/2018

CD200 expression influences neuroinflammatory processes, potentially impacting neurodegenerative diseases.

How does CD200 interact with its receptor CD200R, and what are the implications for immune regulation? 03/18/2018

CD200 interacts with CD200R to modulate immune responses, promoting immune tolerance and reducing inflammation.

Customer Reviews (3)

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Reviews
08/01/2022

    Swift protein sequencing, helps identify unknown proteins.

    05/04/2022

      Great protein crystallization services, key for structural biology.

      05/02/2019

        Invaluable protein-protein interaction assays, integral for research.

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