Recombinant Human PECAM1 cell lysate, His-tagged
Cat.No. : | PECAM1-2798HCL |
Product Overview : | Human Cells transfected lysate in which Human CD31 / PECAM1 has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Human cells |
Species : | Human |
Preparation method : | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80?. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80ºC |
Storage Buffer : | In modified RIPA Lysis Buffer |
Gene Name : | PECAM1 platelet/endothelial cell adhesion molecule 1 [ Homo sapiens ] |
Official Symbol : | PECAM1 |
Synonyms : | PECAM1; platelet/endothelial cell adhesion molecule 1; platelet/endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; CD31; CD31 antigen; PECA1; GPIIA; PECAM-1; endoCAM; CD31/EndoCAM; FLJ34100; FLJ58394; |
Gene ID : | 5175 |
mRNA Refseq : | NM_000442 |
Protein Refseq : | NP_000433 |
MIM : | 173445 |
UniProt ID : | P16284 |
Chromosome Location : | 17q23.3 |
Pathway : | Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Integrin cell surface interactions, organism-specific biosystem; Leukocyte transendothelial migration, organism-specific biosystem; Leukocyte transendothelial migration, conserved biosystem; |
Function : | protein binding; |
Products Types
◆ Recombinant Protein | ||
Pecam1-1179R | Recombinant Rat Pecam1 Protein, His-tagged | +Inquiry |
PECAM1-1073H | Recombinant Human PECAM1 Protein, MYC/DDK-tagged | +Inquiry |
PECAM1-114H | Recombinant Human PECAM1 Protein, C-His-tagged | +Inquiry |
PECAM1-01H | Recombinant Human PECAM1 Protein | +Inquiry |
PECAM1-150H | Recombinant Human PECAM1 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
PECAM1-3050HCL | Recombinant Human PECAM1 cell lysate, Fc-tagged | +Inquiry |
PECAM1-2266MCL | Recombinant Mouse PECAM1 cell lysate | +Inquiry |
PECAM1-3049HCL | Recombinant Human PECAM1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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