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Recombinant Human MSR1 cell lysate

Cat.No. : MSR1-2844HCL
Product Overview : Human MSR1 / SCARA1 / CD204 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : MSR1 macrophage scavenger receptor 1 [ Homo sapiens ]
Official Symbol : MSR1
Synonyms : MSR1; macrophage scavenger receptor 1; macrophage scavenger receptor types I and II; CD204; SCARA1; scavenger receptor class A member 1; scavenger receptor class A, member 1; macrophage scavenger receptor type III; macrophage acetylated LDL receptor I and II; SRA; SR-A; phSR1; phSR2;
Gene ID : 4481
mRNA Refseq : NM_138716
Protein Refseq : NP_619730
MIM : 153622
UniProt ID : P21757
Chromosome Location : 8p22
Pathway : Phagosome, organism-specific biosystem; Phagosome, conserved biosystem;
Function : low-density lipoprotein particle binding; protein binding; receptor activity; scavenger receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What is the primary function of the MSR1 protein, and in which cellular processes does it play a crucial role? 09/24/2020

MSR1 (Macrophage Scavenger Receptor 1) is involved in the recognition and clearance of modified low-density lipoproteins (LDL) by macrophages, playing a critical role in lipid homeostasis.

How does MSR1 contribute to the resolution of inflammation, and what mechanisms are involved in its anti-inflammatory effects? 02/10/2020

MSR1 contributes to the resolution of inflammation by promoting the clearance of apoptotic cells and debris. Its anti-inflammatory effects involve modulating cytokine production and signaling.

What role does MSR1 play in antigen presentation and T-cell activation within the immune system? 06/21/2019

MSR1 participates in antigen presentation by macrophages, influencing T-cell activation. This role connects MSR1 to adaptive immune responses.

What is the relationship between MSR1 and the clearance of amyloid-beta peptides, and how does this link to neurodegenerative diseases? 10/13/2018

MSR1 is involved in the clearance of amyloid-beta peptides associated with neurodegenerative diseases such as Alzheimer's. Its role in phagocytosis and clearance is crucial in the context of neuroinflammation.

How does MSR1 contribute to the immune response, and what are its implications in the context of inflammation and atherosclerosis? 01/31/2018

MSR1 modulates the immune response by regulating inflammatory signaling pathways. Its involvement in recognizing modified LDL in atherosclerotic plaques links it to inflammation and atherosclerosis.

Customer Reviews (3)

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Reviews
03/19/2022

    No more worries about protein sourcing. This product is a lifesaver in the lab.

    07/03/2020

      Outstanding service! Quick response to inquiries and prompt shipping. A+

      10/26/2019

        This is a must-have in any lab. Consistent quality and great results every time.

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