Recombinant Human CTSS cell lysate
Cat.No. : | CTSS-3021HCL |
Product Overview : | Human CTSS / Cathepsin-S derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | CTSS cathepsin S [ Homo sapiens ] |
Official Symbol : | CTSS |
Synonyms : | CTSS; cathepsin S; MGC3886; FLJ50259; |
Gene ID : | 1520 |
mRNA Refseq : | NM_001199739 |
Protein Refseq : | NP_001186668 |
MIM : | 116845 |
UniProt ID : | P25774 |
Chromosome Location : | 1q21 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; Endosomal/Vacuolar pathway, organism-specific biosystem; |
Function : | cysteine-type endopeptidase activity; peptidase activity; |
Products Types
◆ Recombinant Protein | ||
CTSS-1329R | Recombinant Rat CTSS Protein, His (Fc)-Avi-tagged | +Inquiry |
CTSS-2118H | Recombinant Human CTSS Protein, GST-tagged | +Inquiry |
CTSS-282H | Active Recombinant Human CTSS protein(Met1-Ile331), His-tagged | +Inquiry |
Ctss-1293R | Recombinant Rat Ctss Protein, His-tagged | +Inquiry |
CTSS-2695H | Active Recombinant Human CTSS protein, His-tagged | +Inquiry |
◆ Native Protein | ||
CTSS-27405TH | Native Human CTSS | +Inquiry |
◆ Lysates | ||
CTSS-1563MCL | Recombinant Mouse CTSS cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-2353 | Cathepsin S Inhibitor Screening Kit (Fluorometric) | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (6)
Ask a questionThe function of CTSS proteins can be studied through gene knockout or knockdown techniques, such as using techniques such as CRISPR-Cas9 or RNAi. These techniques can create cellular models of missing or reduced CTSS proteins to observe their effects on cell death, among other things.
Expression level of CTSS protein can be detected by immunohistochemistry, western blotting, and real-time quantitative PCR.
CTSS proteins promote cell death by degrading proteins inside cells. During apoptosis, CTSS proteins degrade key proteins within cells, leading to cell death.
The interaction between CTSS proteins and other proteins is regulated by the formation of complexes or the regulation of signal transduction pathways. These interactions can be influenced by a variety of factors, such as ion concentrations, neurotransmitters, and growth factors.
Some studies have shown that the expression level of CTSS protein may be associated with the onset and progression of certain diseases. For example, in some cancers, the expression level of the CTSS protein may change.
It is mainly expressed in lysosomes, which is one of the major proteases in lysosomes.
Customer Reviews (3)
Write a reviewHigh catalytic efficiency, short time, saving time.
The solubility is very good and can be easily dissolved in various buffers.
Anti-pollution ability is very strong, not easy to be polluted.
Ask a Question for All CTSS Products
Required fields are marked with *
My Review for All CTSS Products
Required fields are marked with *
Inquiry Basket