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Recombinant Human GMCSF/IL-3 Fusion Protein

Cat. No.: IL3-88H
Product Overview: Recombinant Human GMCSF/IL3 fusion protein produced in E.Coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kD.
Description: The analog of GMCSF/IL3 is PIXY321. Results indicate that PIXY321 can stimulate multilineage hematopoiesis in vivo and enhance neutrophil and platelet recovery following chemotherapy and bone marrow transplantation (BMT). These results suggest that PIXY321 elicits the biological effects of both its component cytokines and represents a novel means of delivering two independent but interactive cytokines in combination. PIXY321 is purified by proprietary chromatographic techniques.
Synonyms: PIXY321; GMCSF/ IL3
Source: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (c) Analysis by SDS-PAGE.
Formulation: The protein was lyophilized after extensive dialysis against 10 mM PBS, pH= 7.4±0.1 and 0.1% BSA.
Solubility: It is recommended to reconstitute the lyophilized PIXY321 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.2 ng/ml, corresponding to a Specific Activity of 5 x106IU/mg.
Stability: Lyophilized PIXY321although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution PIXY321should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.

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