Recombinant Mouse Csf1

• Cat.No.: Csf1-95M
• Product Overview: Recombinant Macrophage Colony Stimulating Factor produced inE.coliis a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD. MCSF is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: Csf1-95M
• Description: Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
• Source: Escherichia Coli.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Lys-Glu-Val-Ser.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein (1 mg/ml) was lyophilized with 5mM Tris, pH 8.0 + 100 mM NaCl.
• Solubility: It is recommended to reconstitute the lyophilized M-CSF in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED50, calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells was found < 1 ng/ml, corresponding to a Specific Activity of 1 x 10⁶ IU/mg.
• Stability: Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°Cbetween 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Mus musculus ]
• Synonyms: Csf1; colony stimulating factor 1 (macrophage); op; Csfm; CSF-1; M-CSF; C87615; colony stimulating factor 1; osteopetrosis; colony-stimulating factor-1
• Gene ID: 12977
• mRNA Refseq: NM_001113529
• Protein Refseq: NP_001107001
• UniProt ID: P07141
• Chromosome Location: 3 F3; 3 51.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage
• Function: cytokine activity; growth factor activity; macrophage colony stimulating factor receptor binding; protein binding