"WNT3" Related Products

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Recombinant Human WNT3 293 Cell Lysate

Cat.No.: WNT3-296HCL
Description: Antigen standard for wingless-type MMTV integration site family, member 3 (WNT3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: WNT3 wingless-type MMTV integration site family, member 3 [ Homo sapiens ]
Official Symbol: WNT3
Synonyms: WNT3; wingless-type MMTV integration site family, member 3; INT4; proto-oncogene Wnt-3; MGC131950; MGC138321; MGC138323; WNT 3 proto oncogene protein; WNT-3 proto-oncogene protein; proto-oncogene Int-4 homolog;
Gene ID: 7473
mRNA Refseq: NM_030753
Protein Refseq: NP_110380
MIM: 165330
UniProt ID: P56703
Chromosome Location: 17q21-q22
Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem;

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