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Recombinant Human UBE2D2 293 Cell Lysate

Cat.No. : UBE2D2-588HCL
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  • Gene Information
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Description : Antigen standard for ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) (UBE2D2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : UBE2D2 ubiquitin-conjugating enzyme E2D 2 [ Homo sapiens ]
Official Symbol : UBE2D2
Synonyms : UBE2D2; ubiquitin-conjugating enzyme E2D 2; ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) , ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2 D2; UBC4; UbcH5B; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2(17)KB 2; ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); PUBC1; UBC4/5; UBCH5B; E2(17)KB2;
Gene ID : 7322
mRNA Refseq : NM_181838
Protein Refseq : ?NP_862821
MIM : 602962
UniProt ID : P62837
Chromosome Location : 5q31.3
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does UBE2D2 interact with other components of the ubiquitin-proteasome system? 02/19/2023

UBE2D2 works in tandem with other ubiquitin-proteasome system components, orchestrating protein degradation.

How does UBE2D2 contribute to cellular protein degradation? 12/27/2020

It facilitates the attachment of ubiquitin to target proteins, directing them to the proteasome for breakdown.

What role does UBE2D2 play in cell cycle regulation? 11/09/2019

UBE2D2 plays a role in controlling the cell cycle by regulating the degradation of key cycle-related proteins.

What potential therapeutic applications arise from targeting UBE2D2 in diseases related to protein misfolding? 11/24/2018

Targeting UBE2D2 could offer new treatments for conditions involving protein misfolding and aggregation.

What is the impact of altered UBE2D2 activity on disease development? 06/22/2017

Altered UBE2D2 activity is linked with several diseases, including cancer and neurodegenerative disorders.

How do genetic variations in UBE2D2 affect cellular homeostasis? 06/12/2017

Genetic mutations in UBE2D2 can disrupt protein turnover, affecting various cellular functions and stability.

What is the primary role of UBE2D2 in protein ubiquitination? 03/14/2017

UBE2D2, a ubiquitin-conjugating enzyme, is vital for tagging proteins with ubiquitin, marking them for degradation.

Customer Reviews (3)

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07/12/2022

    Trusted for research integrity, supports research consistency.

    10/24/2020

      Outstanding accuracy, highly recommended for insights.

      12/30/2017

        Efficient service, accelerates our research timelines.

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