Recombinant Human UBE2D2 293 Cell Lysate
Cat.No. : | UBE2D2-588HCL |
- Specification
- Gene Information
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Description : | Antigen standard for ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast) (UBE2D2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | UBE2D2 ubiquitin-conjugating enzyme E2D 2 [ Homo sapiens ] |
Official Symbol : | UBE2D2 |
Synonyms : | UBE2D2; ubiquitin-conjugating enzyme E2D 2; ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) , ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2 D2; UBC4; UbcH5B; ubiquitin-protein ligase D2; ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2(17)KB 2; ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); PUBC1; UBC4/5; UBCH5B; E2(17)KB2; |
Gene ID : | 7322 |
mRNA Refseq : | NM_181838 |
Protein Refseq : | ?NP_862821 |
MIM : | 602962 |
UniProt ID : | P62837 |
Chromosome Location : | 5q31.3 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; |
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UBE2D2-243H | Recombinant Human UBE2D2 Protein, His-tagged | +Inquiry |
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◆ Lysates | ||
UBE2D2-587HCL | Recombinant Human UBE2D2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionUBE2D2 works in tandem with other ubiquitin-proteasome system components, orchestrating protein degradation.
It facilitates the attachment of ubiquitin to target proteins, directing them to the proteasome for breakdown.
UBE2D2 plays a role in controlling the cell cycle by regulating the degradation of key cycle-related proteins.
Targeting UBE2D2 could offer new treatments for conditions involving protein misfolding and aggregation.
Altered UBE2D2 activity is linked with several diseases, including cancer and neurodegenerative disorders.
Genetic mutations in UBE2D2 can disrupt protein turnover, affecting various cellular functions and stability.
UBE2D2, a ubiquitin-conjugating enzyme, is vital for tagging proteins with ubiquitin, marking them for degradation.
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