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Recombinant Human TRIM33 293 Cell Lysate

Cat.No. : TRIM33-781HCL
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  • Gene Information
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Description : Antigen standard for tripartite motif-containing 33 (TRIM33), transcript variant a is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : TRIM33 tripartite motif containing 33 [ Homo sapiens ]
Official Symbol : TRIM33
Synonyms : TRIM33; tripartite motif containing 33; E3 ubiquitin-protein ligase TRIM33; FLJ11429; KIAA1113; PTC7; ret fused gene 7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA; transcriptional intermediary factor 1 gamma; TIF1-gamma; protein Rfg7; ectodermin homolog; RET-fused gene 7 protein; tripartite motif-containing 33; ECTO; FLJ32925;
Gene ID : 51592
mRNA Refseq : NM_015906
Protein Refseq : NP_056990
MIM : 605769
UniProt ID : Q9UPN9
Chromosome Location : 1p13.1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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