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Recombinant Human SQSTM1 293 Cell Lysate

Cat.No.: SQSTM1-1481HCL
Description: Antigen standard for sequestosome 1 (SQSTM1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SQSTM1 sequestosome 1 [ Homo sapiens ]
Official Symbol: SQSTM1
Synonyms: SQSTM1; sequestosome 1; OSIL, oxidative stress induced like , Paget disease of bone 3 , PDB3; sequestosome-1; A170; p60; p62; p62B; EBIAP; EBI3-associated protein p60; oxidative stress induced like; ubiquitin-binding protein p62; EBI3-associated protein of 60 kDa; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; OSIL; PDB3; ZIP3;
Gene ID: 8878
mRNA Refseq: NM_001142298
Protein Refseq: NP_001135770
MIM: 601530
UniProt ID: Q13501
Chromosome Location: 5q35
Pathway: Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL1-mediated signaling events, organism-specific biosystem; Immune System, organism-specific biosystem; Interleukin-1 signaling, organism-specific biosystem; NF-kB is activated and signals survival, organism-specific biosystem; NRIF signals cell death from the nucleus, organism-specific biosystem;

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