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Recombinant Human SPAM1 293 Cell Lysate

Cat.No.: SPAM1-1546HCL
Description: Antigen standard for sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) (SPAM1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ]
Official Symbol: SPAM1
Synonyms: SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); hyaluronidase PH-20; HYAL5; PH 20; SPAG15; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; HYA1; PH20; HYAL1; HYAL3; PH-20; MGC26532;
Gene ID: 6677
mRNA Refseq: NM_003117
Protein Refseq: NP_003108
MIM: 600930
UniProt ID: P38567
Chromosome Location: 7q31
Pathway: Chondroitin sulfate degradation, organism-specific biosystem; Chondroitin sulfate degradation, conserved biosystem; Dermatan sulfate degradation, organism-specific biosystem; Dermatan sulfate degradation, conserved biosystem; Glycosaminoglycan degradation, organism-specific biosystem; Glycosaminoglycan degradation, conserved biosystem; Metabolic pathways, organism-specific biosystem;
Function: catalytic activity; hyalurononglucosaminidase activity; hydrolase activity, acting on glycosyl bonds;

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