Recombinant Mouse Spam1 Protein, His-tagged

• Cat.No.: Spam1-2092M
• Product Overview: Recombinant Mouse Spam1 Protein (Val36-Cys464) with N-His tag was expressed in E. coli.

Specification

• Description: Enables hyalurononglucosaminidase activity. Acts upstream of or within single fertilization. Located in acrosomal vesicle; external side of plasma membrane; and membrane raft. Orthologous to human SPAM1 (sperm adhesion molecule 1).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 52.8 kDa
  Accurate Molecular Mass: 53 kDa
• Protein length: Val36-Cys464
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Spam1 sperm adhesion molecule 1 [ Mus musculus (house mouse) ]
• Official Symbol: Spam1
• Synonyms: SPAM1; sperm adhesion molecule 1; hyaluronidase PH-20; hyal-PH20; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20; Ph-20; AV039194
• Gene ID: 20690
• mRNA Refseq: NM_001079875
• Protein Refseq: NP_001073344
• UniProt ID: P48794