"SOCS3" Related Products


Recombinant Human SOCS3 293 Cell Lysate

Cat.No.: SOCS3-1580HCL
Description: Antigen standard for suppressor of cytokine signaling 3 (SOCS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SOCS3 suppressor of cytokine signaling 3 [ Homo sapiens ]
Official Symbol: SOCS3
Synonyms: SOCS3; suppressor of cytokine signaling 3; CIS3; Cish3; SOCS 3; SSI 3; CIS-3; STAT-induced STAT inhibitor 3; cytokine-induced SH2 protein 3; cytokine-inducible SH2 protein 3; SSI3; ATOD4; SSI-3; SOCS-3; MGC71791;
Gene ID: 9021
mRNA Refseq: NM_003955
Protein Refseq: NP_003946
MIM: 604176
UniProt ID: O14543
Chromosome Location: 17q25.3
Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem;
Function: protein kinase inhibitor activity;

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