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Recombinant Human SLCO1B3 293 Cell Lysate

Cat.No.: SLCO1B3-1688HCL
Description: Antigen standard for solute carrier organic anion transporter family, member 1B3 (SLCO1B3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SLCO1B3 solute carrier organic anion transporter family, member 1B3 [ Homo sapiens ]
Official Symbol: SLCO1B3
Synonyms: SLCO1B3; solute carrier organic anion transporter family, member 1B3; SLC21A8, solute carrier family 21 (organic anion transporter), member 8; solute carrier organic anion transporter family member 1B3; OATP1B3; OATP8; organic anion transporter 8; organic anion transporter LST-3c; organic anion-transporting polypeptide 8; liver-specific organic anion transporter 2; liver-specific organic anion transporter 3TM13; solute carrier family 21 (organic anion transporter), member 8; LST3; HBLRR; LST-2; OATP-8; SLC21A8; LST-3TM13;
Gene ID: 28234
mRNA Refseq: NM_019844
Protein Refseq: NP_062818
MIM: 605495
UniProt ID: Q9NPD5
Chromosome Location: 12p12
Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Recycling of bile acids and salts, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem;
Function: organic anion transmembrane transporter activity; transporter activity;

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