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Recombinant Human SEPT4 293 Cell Lysate

Cat.No. : SEPT4-1958HCL
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  • Gene Information
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Description : Antigen standard for septin 4 (SEPT4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SEPT4 septin 4 [ Homo sapiens ]
Official Symbol : SEPT4
Synonyms : H5; ARTS; MART; SEP4; CE5B3; PNUTL2; hucep-7; BRADEION; hCDCREL-2; septin-4; septin-M; CE5B3 beta; bradeion beta; brain protein H5; cerebral protein 7; peanut-like protein 2; cell division control-related protein 2; apoptosis-related protein in the TGF-beta signaling pathway
Gene ID : 5414
mRNA Refseq : NM_080415
Protein Refseq : NP_536340
MIM : 603696
UniProt ID : O43236
Chromosome Location : 17q22
Pathway : Apoptosis Modulation and Signaling, organism-specific biosystem
Function : GTP binding; protein binding; structural molecule activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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