"SAR1A" Related Products

Recombinant Human SAR1A 293 Cell Lysate

Cat.No.: SAR1A-2065HCL
Description: Antigen standard for SAR1 homolog A (S. cerevisiae) (SAR1A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SAR1A SAR1 homolog A (S. cerevisiae) [ Homo sapiens ]
Official Symbol: SAR1A
Synonyms: SAR1A; SAR1 homolog A (S. cerevisiae); SAR1a gene homolog (S. cerevisiae) 1 , SAR1a gene homolog 1 (S. cerevisiae) , SARA1; GTP-binding protein SAR1a; SAR1; Sara; SAR1 gene homolog A; SAR1a gene homolog 1; COPII-associated small GTPase; SARA1; masra2;
Gene ID: 56681
mRNA Refseq: NM_001142648
Protein Refseq: NP_001136120
MIM: 607691
UniProt ID: Q9NR31
Chromosome Location: 10q22.1
Pathway: COPII complex, organism-specific biosystem; Legionellosis, organism-specific biosystem; Legionellosis, conserved biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
Function: GTP binding; GTPase activity; nucleotide binding;

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