||Recombinant Human CSRC produced inSf9 cellsis a glycosylated, polypeptide chain having a molecular mass of 60 KD. CSRC is purified by proprietary chromatographic techniques.
||SRC family kinases are involved in transducing growth factor signals for cellular differentiation and proliferation in a variety of cell types. The activity of all Src family kinases (SFKs) is controlled by phosphorylation at their C-terminal 527-tyrosine residue by C-terminal SRC kinase, CSK. The C-terminal SRC kinase (CSK) family of protein tyrosine kinases contains two members: CSK and CSK homologous kinase (CHK). Both phosphorylate and inactivate SRC family kinases. C-terminal SRC kinase (CSK) contains a catalytic domain and a regulatory region, consisting of an SH3 and an SH2 domain.
||Sf9 Insect Cells.
||Sterile Filtered clear solution.
||Greater than 95% as determined by SDS-PAGE.
||C-SRC is supplied at a concentration of 0.29mg/ml in 50mM Tris, pH 7.6, 0.15 M NaCl, 0.27M sucrose, 1mM DTT, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 5mM NaF, 10mM β-glycerol phosphate, 0.1mM sodium vanadate and 0.03% Brij-35.
||750 units/mg. One unit of C-SRC activity is equal to 1 nmol of phosphate transferred to a peptide corresponding to p34cdc2 (6-20) per minute at 30°C with a final ATP concentration of 100 µM. Recombinant human active C-SRC also phosphorylates STAT, p34cdc2, gastrin, and cortactin.Please note: Kinase activity may vary depending on the substrate and reaction conditions.
||Store at 4°C if entire vial will be used within 1-2 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.