"RPF2" Related Products

Recombinant Human RPF2 293 Cell Lysate

Cat.No.: RPF2-2235HCL
Description: Antigen standard for ribosome production factor 2 homolog (S. cerevisiae) (RPF2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RPF2 ribosome production factor 2 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: RPF2
Synonyms: RPF2; ribosome production factor 2 homolog (S. cerevisiae); brix domain containing 1 , BXDC1; ribosome production factor 2 homolog; bA397G5.4; FLJ21087; ribosomal processing factor 2 homolog (S. cerevisiae); homolog of Rpf2; brix domain containing 1; brix domain-containing protein 1; ribosomal processing factor 2 homolog; ribosome biogenesis protein RPF2 homolog; BXDC1;
Gene ID: 84154
mRNA Refseq: NM_032194
Protein Refseq: NP_115570
UniProt ID: Q9H7B2
Chromosome Location: 6q21

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