||Recombinant Human EGFR encoding a.a. 672-1210 expressed in Baculovirus infectedSf9 cells. EGFR is purified by proprietary chromatographic techniques.
||The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1 or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoprotein that has an extracellular domain which contains two cysteine-rich domains separated by a spacer region that is involved in ligand-binding, and a cytoplasmic domain which has a membrane-proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGF R gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF-á, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 in the absence of a co-receptor. Ligand binding induces EGF R homodimerization as well as heterdimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling.
||Baculovirus infected Sf9 cells.
||Sterile filtered liquid.
||EGFR (0.16 mg/ml) in 50mM Tris-HCl, pH 8.0, 100mM NaCl, 5mM DTT, 15mM reduced glutathione and 20% glycerol.
|Application And Suggested Dilutions:
||• ELISA • Inhibition Assays • Western Blotting.
||Determination of Km value by Filter binding assay MAFC membrane. Specific activity: 23.000 pmol/mg min.
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 91 kDa.
||Store vial at -20ºC to -80ºC. When stored at the recommended temperature, this protein is stable for 12 months. Please avoid freeze-thaw cycles.