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Recombinant Human RAGE 293 Cell Lysate

Cat.No. : AGER-2546HCL
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  • Gene Information
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Description : Antigen standard for renal tumor antigen (RAGE) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : AGER advanced glycosylation end product-specific receptor [ Homo sapiens ]
Official Symbol : AGER
Synonyms : RAGE; advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta; receptor for advanced glycation end-products variant 20
Gene ID : 177
mRNA Refseq : http://www.ncbi.nlm.nih.gov/nuccore/NM_001136.4
Protein Refseq : NP_001127
MIM : 600214
UniProt ID : Q15109
Chromosome Location : 6p21.3
Pathway : AGE/RAGE pathway, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; Innate Immune System, organism-specific biosystem
Function : S100 protein binding; identical protein binding; receptor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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How does AGER affect bone health? 09/02/2022

AGER is involved in bone metabolism and might influence osteoporosis and bone diseases.

Are there diagnostic applications for AGER? 08/10/2022

AGER levels might serve as biomarkers for certain diseases, including diabetes.

How does AGER interact with AGEs? 07/05/2022

AGER binds AGEs, initiating signaling cascades linked to inflammation, oxidative stress, and tissue damage.

How is AGER implicated in skin aging? 05/30/2022

AGER contributes to skin aging through oxidative stress and collagen damage.

What is the AGER protein? 07/15/2021

AGER stands for "Advanced Glycosylation End-Product Specific Receptor," a cell surface receptor.

Can the AGER protein impact inflammation? 07/15/2021

Yes, AGER activation contributes to inflammation and immune responses.

How does AGER impact wound healing? 02/05/2021

AGER activation can impair wound healing through inflammation and oxidative stress.

How does AGER influence neurodegenerative diseases? 01/22/2021

AGER contributes to neuroinflammation and might play a role in neurodegeneration.

Can AGER influence kidney function? 12/06/2020

AGER contributes to diabetic nephropathy and kidney dysfunction.

How does the AGER protein interact with AGEs? 09/23/2020

AGER binds to AGEs, initiating cellular signaling pathways linked to inflammation and oxidative stress.

Can AGER influence lung health? 01/31/2020

AGER is implicated in lung diseases and inflammation, such as chronic obstructive pulmonary disease.

Are there therapeutic strategies targeting AGER? 06/09/2019

Researchers explore compounds that modulate AGER to mitigate its effects.

How is AGER involved in diabetic complications? 05/13/2019

AGER contributes to diabetic complications by promoting inflammation and tissue damage.

How is the AGER protein involved in research? 01/28/2019

AGER is studied for its implications in aging, diabetes, and various age-related diseases.

What is the function of the AGER protein? 07/25/2018

AGER plays a role in mediating cellular responses to advanced glycation end products (AGEs).

Are there dietary interventions for AGER-related effects? 10/05/2017

Consuming a low-AGE diet may help reduce AGER activation and its negative effects.

Can AGER impact immune responses? 06/29/2017

AGER activation affects immune cell functions and influences immune responses.

Does AGER affect cardiovascular health? 12/04/2016

AGER activation is linked to cardiovascular diseases, including atherosclerosis.

Are there other ligands for the AGER protein? 11/25/2016

Yes, besides AGEs, AGER interacts with other ligands like HMGB1 and β-amyloid.

Is AGER research ongoing? 11/02/2016

Yes, ongoing research aims to uncover new roles and therapeutic targets related to AGER.

Customer Reviews (5)

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Reviews
02/26/2022

    It's quality was evident in consistent results across replicates.

    08/13/2021

       Protein consistently upregulated the expression of tissue-specific markers.

      01/20/2020

         Gel electrophoresis bands displayed distinct separation, showcasing the product’s purity.

        06/22/2019

          It’s consistent outcomes supported reliable statistical analysis.

          01/11/2017

            Consistency and Compatibility Consistency in the product’s performance bolstered the credibility of our research findings.

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