Recombinant Human RAGE 293 Cell Lysate
Cat.No. : | AGER-2546HCL |
- Specification
- Gene Information
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Description : | Antigen standard for renal tumor antigen (RAGE) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AGER advanced glycosylation end product-specific receptor [ Homo sapiens ] |
Official Symbol : | AGER |
Synonyms : | RAGE; advanced glycosylation end product-specific receptor; RAGE isoform sRAGE-delta; RAGE isoform NtRAGE-delta; receptor for advanced glycation end-products variant 20 |
Gene ID : | 177 |
mRNA Refseq : | http://www.ncbi.nlm.nih.gov/nuccore/NM_001136.4 |
Protein Refseq : | NP_001127 |
MIM : | 600214 |
UniProt ID : | Q15109 |
Chromosome Location : | 6p21.3 |
Pathway : | AGE/RAGE pathway, organism-specific biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; Innate Immune System, organism-specific biosystem |
Function : | S100 protein binding; identical protein binding; receptor activity |
Products Types
◆ Lysates | ||
AGER-2215MCL | Recombinant Mouse AGER cell lysate | +Inquiry |
AGER-1480HCL | Recombinant Human AGER cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (20)
Ask a questionAGER is involved in bone metabolism and might influence osteoporosis and bone diseases.
AGER levels might serve as biomarkers for certain diseases, including diabetes.
AGER binds AGEs, initiating signaling cascades linked to inflammation, oxidative stress, and tissue damage.
AGER contributes to skin aging through oxidative stress and collagen damage.
AGER stands for "Advanced Glycosylation End-Product Specific Receptor," a cell surface receptor.
Yes, AGER activation contributes to inflammation and immune responses.
AGER activation can impair wound healing through inflammation and oxidative stress.
AGER contributes to neuroinflammation and might play a role in neurodegeneration.
AGER contributes to diabetic nephropathy and kidney dysfunction.
AGER binds to AGEs, initiating cellular signaling pathways linked to inflammation and oxidative stress.
AGER is implicated in lung diseases and inflammation, such as chronic obstructive pulmonary disease.
Researchers explore compounds that modulate AGER to mitigate its effects.
AGER contributes to diabetic complications by promoting inflammation and tissue damage.
AGER is studied for its implications in aging, diabetes, and various age-related diseases.
AGER plays a role in mediating cellular responses to advanced glycation end products (AGEs).
Consuming a low-AGE diet may help reduce AGER activation and its negative effects.
AGER activation affects immune cell functions and influences immune responses.
AGER activation is linked to cardiovascular diseases, including atherosclerosis.
Yes, besides AGEs, AGER interacts with other ligands like HMGB1 and β-amyloid.
Yes, ongoing research aims to uncover new roles and therapeutic targets related to AGER.
Customer Reviews (5)
Write a reviewIt's quality was evident in consistent results across replicates.
Protein consistently upregulated the expression of tissue-specific markers.
Gel electrophoresis bands displayed distinct separation, showcasing the product’s purity.
It’s consistent outcomes supported reliable statistical analysis.
Consistency and Compatibility Consistency in the product’s performance bolstered the credibility of our research findings.
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