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Recombinant Human PTGR1 293 Cell Lysate

Cat.No.: PTGR1-2708HCL
Description: Antigen standard for prostaglandin reductase 1 (PTGR1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PTGR1 prostaglandin reductase 1 [ Homo sapiens ]
Official Symbol: PTGR1
Synonyms: PTGR1; prostaglandin reductase 1; leukotriene B4 12 hydroxydehydrogenase , LTB4DH; ZADH3; zinc binding alcohol dehydrogenase domain containing 3; PRG-1; 15-oxoprostaglandin 13-reductase; leukotriene B4 12-hydroxydehydrogenase; NADP-dependent leukotriene B4 12-hydroxydehydrogenase; PGR1; LTB4DH; FLJ99229; MGC34943;
Gene ID: 22949
mRNA Refseq: NM_001146108
Protein Refseq: NP_001139580
MIM: 601274
UniProt ID: Q14914
Chromosome Location: 9q32
Function: 15-oxoprostaglandin 13-oxidase activity; 2-alkenal reductase [NAD(P)] activity; alcohol dehydrogenase (NAD) activity; nucleotide binding; oxidoreductase activity; zinc ion binding;

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