Recombinant Human PTGIS 293 Cell Lysate

• Cat.No.: PTGIS-2709HCL

Specification

• Description: Antigen standard for prostaglandin I2 (prostacyclin) synthase (PTGIS) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PTGIS prostaglandin I2 (prostacyclin) synthase [ Homo sapiens ]
• Official Symbol: PTGIS
• Synonyms: PTGIS; prostaglandin I2 (prostacyclin) synthase; prostacyclin synthase; CYP8A1; cytochrome P450; family 8; subfamily A; polypeptide 1; PGIS; prostaglandin I2 synthase; cytochrome P450, family 8, subfamily A, polypeptide 1; CYP8; PTGI; MGC126858; MGC126860
• Gene ID: 5740
• mRNA Refseq: NM_000961
• Protein Refseq: NP_000952
• MIM: 601699
• UniProt ID: Q16647
• Chromosome Location: 20q13
• Pathway: Adipogenesis, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Eicosanoid Synthesis, organism-specific biosystem; Eicosanoids, organism-specific biosystem
• Function: electron carrier activity; heme binding; isomerase activity; metal ion binding; monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; prostaglandin-I synthase activity