"PPM1L" Related Products

Recombinant Human PPM1L 293 Cell Lysate

Cat.No.: PPM1L-2956HCL
Description: Antigen standard for protein phosphatase 1 (formerly 2C)-like (PPM1L) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPM1L protein phosphatase, Mg2+/Mn2+ dependent, 1L [ Homo sapiens ]
Official Symbol: PPM1L
Synonyms: PPM1L; protein phosphatase, Mg2+/Mn2+ dependent, 1L; protein phosphatase 1 (formerly 2C) like; protein phosphatase 1L; PP2CE; PP2Cepsilon; Protein phosphatase 2C epsilon isoform; PP2C epsilon; protein phosphatase 2C isoform epsilon; PPM1-LIKE; PP2C-epsilon; MGC132545; MGC132547;
Gene ID: 151742
mRNA Refseq: NM_139245
Protein Refseq: NP_640338
MIM: 611931
UniProt ID: Q5SGD2
Chromosome Location: 3q26.1
Pathway: Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem;
Function: hydrolase activity; metal ion binding; protein serine/threonine phosphatase activity;

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