Recombinant Human PPM1G 293 Cell Lysate

• Cat.No.: PPM1G-2959HCL

Specification

• Description: Antigen standard for protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform (PPM1G), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPM1G protein phosphatase, Mg2+/Mn2+ dependent, 1G [ Homo sapiens ]
• Official Symbol: PPM1G
• Synonyms: PPM1G; protein phosphatase, Mg2+/Mn2+ dependent, 1G; protein phosphatase 1G (formerly 2C), magnesium dependent, gamma isoform; protein phosphatase 1G; PP2C; gamma; PP2CG; PP2Cgamma; protein phosphatase 2C; gamma isoform; PP2C-gamma; PP2C, gamma; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; PPP2CG; PP2CGAMMA; MGC1675; MGC2870
• Gene ID: 5496
• mRNA Refseq: NM_002707
• Protein Refseq: NP_002698
• MIM: 605119
• UniProt ID: O15355
• Chromosome Location: 2p23.3
• Pathway: mRNA processing, organism-specific biosystem
• Function: catalytic activity; hydrolase activity; metal ion binding; protein serine/threonine phosphatase activity

Reviews

11/14/2022

PPM1G protein's utility in protein electron microscopy structure analysis is commendable.

12/03/2019

It enables researchers to delve deeper into the intricacies of protein behavior and aids in advancing our knowledge in various fields, such as molecular biology, biochemistry, and structural biology.

11/11/2019

It demonstrates excellent performance in ELISA assays, making it an ideal choice for researchers studying protein-protein interactions, antibody detection, or biomarker identification.

Q&As

Can PPM1G be targeted for cancer treatment?

Research is ongoing to explore the therapeutic potential of targeting PPM1G in cancer. Inhibiting or modulating its activity may be a strategy for cancer therapy.

Are there any known associations between PPM1G and cancer?

Yes, abnormal expression of PPM1G has been linked to certain cancers, suggesting its potential role as a biomarker or therapeutic target.

Are there any genetic disorders associated with PPM1G mutations?

While rare, mutations in the PPM1G gene have been reported and may be associated with certain genetic disorders. Further research is needed to understand the implications.

How does PPM1G contribute to DNA repair mechanisms?

PPM1G is involved in the regulation of DNA repair processes, influencing the repair of damaged DNA and maintaining genomic stability.

Can PPM1G be used as a diagnostic marker for specific diseases?

PPM1G expression levels may serve as a diagnostic marker for certain diseases, including some cancers, but more studies are required to establish its diagnostic utility.