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Recombinant Human POR 293 Cell Lysate

Cat.No.: POR-3006HCL
Description: Antigen standard for P450 (cytochrome) oxidoreductase (POR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: POR P450 (cytochrome) oxidoreductase [ Homo sapiens ]
Official Symbol: POR
Synonyms: POR; P450 (cytochrome) oxidoreductase; NADPH--cytochrome P450 reductase; CYPOR; FLJ26468; NADPH-dependent cytochrome P450 reductase; CPR; P450R; DKFZp686G04235;
Gene ID: 5447
mRNA Refseq: NM_000941
Protein Refseq: NP_000932
MIM: 124015
UniProt ID: P16435
Chromosome Location: 7q11.2
Pathway: 1,25-dihydroxyvitamin D3 biosynthesis, organism-specific biosystem; 1,25-dihydroxyvitamin D3 biosynthesis, conserved biosystem; melatonin degradation I, organism-specific biosystem; melatonin degradation I, conserved biosystem; superpathway of melatonin degradation, conserved biosystem; superpathway of melatonin degradation, organism-specific biosystem;
Function: FMN binding; NADP binding; NADPH-hemoprotein reductase activity; NADPH-hemoprotein reductase activity; cytochrome-b5 reductase activity; electron carrier activity; enzyme binding; flavin adenine dinucleotide binding; hydrolase activity; iron ion binding; iron-cytochrome-c reductase activity; oxidoreductase activity;

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