"POLR3H" Related Products

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Recombinant Human POLR3H 293 Cell Lysate

Cat.No.: POLR3H-3022HCL
Description: Antigen standard for polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) (POLR3H), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: POLR3H polymerase (RNA) III (DNA directed) polypeptide H (22.9kD) [ Homo sapiens ]
Official Symbol: POLR3H
Synonyms: POLR3H; polymerase (RNA) III (DNA directed) polypeptide H (22.9kD); DNA-directed RNA polymerase III subunit RPC8; KIAA1665; RPC8; RNA polymerase III subunit C8; RNA polymerase III subunit RPC8; DNA-directed RNA polymerase III subunit H; RNA nucleotidyltransferase (DNA-directed); RNA polymerase III subunit 22.9 kDa subunit; DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide; RPC22.9; MGC29654; MGC111097;
Gene ID: 171568
mRNA Refseq: NM_001018050
Protein Refseq: NP_001018060
UniProt ID: Q9Y535
Chromosome Location: 22q13
Pathway: Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Epstein-Barr virus infection, organism-specific biosystem; Epstein-Barr virus infection, conserved biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Gene Expression, organism-specific biosystem; Metabolic pathways, organism-specific biosystem;
Function: DNA binding; DNA-directed RNA polymerase activity;

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