Recombinant Human POLR2A 293 Cell Lysate

• Cat.No.: POLR2A-3037HCL

Specification

• Description: Antigen standard for polymerase (RNA) II (DNA directed) polypeptide A, 220kDa (POLR2A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa [ Homo sapiens ]
• Official Symbol: POLR2A
• Synonyms: POLR2A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; POLR2, polymerase (RNA) II (DNA directed) polypeptide A (220kD); DNA-directed RNA polymerase II subunit RPB1; POLRA; RPB1; RNA polymerase II subunit B1; DNA-directed RNA polymerase II subunit A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; RPO2; POLR2; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; MGC75453
• Gene ID: 5430
• mRNA Refseq: NM_000937
• Protein Refseq: NP_000928
• UniProt ID: P24928
• Chromosome Location: 17p13.1
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; Formation of HIV-1 elongation complex containing HIV-1 Tat, organism-specific biosystem; Formation of HIV-1 elongation complex in the absence of HIV-1 Tat, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; RNA-directed RNA polymerase activity; metal ion binding; nucleotidyltransferase activity; protein binding; contributes_to protein binding; contributes_to protein kinase activity; transferase activity; ubiquitin protein ligase binding

Reviews

08/19/2022

manufacturers often have a wealth of knowledge and expertise in the field of POLR2A protein research.

01/30/2022

They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of POLR2A protein in their studies.

12/17/2021

the manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of POLR2A protein in trials.

Q&As

Can POLR2A mutations be used for diagnostic purposes?

Yes, identifying mutations in POLR2A can aid in the diagnosis of certain neurodevelopmental disorders.

Can POLR2A be used as a biomarker for cancer?

Research is ongoing to determine if POLR2A expression can serve as a biomarker for cancer diagnosis or prognosis.

How is POLR2A targeted in cancer therapy?

In cancer treatment, POLR2A is a potential target for the development of therapies that inhibit its function, thereby suppressing cancer cell growth.

What diseases are associated with mutations in POLR2A?

Mutations in POLR2A have been linked to neurodevelopmental disorders, including intellectual disability, autism, and epilepsy.

How does POLR2A function in the context of transcription?

POLR2A is a key component of RNA polymerase II, which transcribes DNA into mRNA, allowing for gene expression and protein synthesis.