"PLSCR1" Related Products


Recombinant Human PLSCR1 293 Cell Lysate

Cat.No.: PLSCR1-3096HCL
Description: Antigen standard for phospholipid scramblase 1 (PLSCR1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLSCR1 phospholipid scramblase 1 [ Homo sapiens ]
Official Symbol: PLSCR1
Synonyms: PLSCR1; phospholipid scramblase 1; MMTRA1B; PL scramblase 1; erythrocyte phospholipid scramblase; ca(2+)-dependent phospholipid scramblase 1;
Gene ID: 5359
mRNA Refseq: NM_021105
Protein Refseq: NP_066928
MIM: 604170
UniProt ID: O15162
Chromosome Location: 3q23
Pathway: EGFR1 Signaling Pathway, organism-specific biosystem;
Function: CD4 receptor binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; SH3 domain binding; calcium ion binding; calcium ion binding; enzyme binding; epidermal growth factor receptor binding; phospholipid scramblase activity; protein binding;

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