"PLA2G15" Related Products


Recombinant Human PLA2G15 293 Cell Lysate

Cat.No.: PLA2G15-3144HCL
Description: Antigen standard for phospholipase A2, group XV (PLA2G15) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLA2G15 phospholipase A2, group XV [ Homo sapiens ]
Official Symbol: PLA2G15
Synonyms: PLA2G15; phospholipase A2, group XV; LYPLA3, lysophospholipase 3 (lysosomal phospholipase A2); group XV phospholipase A2; GXVPLA2; LLPL; 1-O-acylceramide synthase; lysosomal phospholipase A2; LCAT-like lysophospholipase; lysophospholipase 3 (lysosomal phospholipase A2); ACS; LPLA2; LYPLA3; DKFZp564A0122;
Gene ID: 23659
mRNA Refseq: NM_012320
Protein Refseq: NP_036452
MIM: 609362
UniProt ID: Q8NCC3
Chromosome Location: 16q22.1
Pathway: Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem;
Function: O-acyltransferase activity; calcium-independent phospholipase A2 activity; hydrolase activity; lysophospholipase activity; phospholipid binding; transferase activity, transferring acyl groups;

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