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Recombinant Human PIAS1 293 Cell Lysate

Cat.No.: PIAS1-3205HCL
Description: Antigen standard for protein inhibitor of activated STAT, 1 (PIAS1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PIAS1 protein inhibitor of activated STAT, 1 [ Homo sapiens ]
Official Symbol: PIAS1
Synonyms: PIAS1; protein inhibitor of activated STAT, 1; DDXBP1, DEAD/H (Asp Glu Ala Asp/His) box binding protein 1; E3 SUMO-protein ligase PIAS1; GBP; GU/RH II; zinc finger; MIZ type containing 3; ZMIZ3; gu-binding protein; AR interacting protein; DEAD/H box-binding protein 1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3; protein inhibitor of activated STAT-1; protein inhibitor of activated STAT protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DDXBP1; GU/RH-II; MGC141878; MGC141879;
Gene ID: 8554
mRNA Refseq: NM_016166
Protein Refseq: NP_057250
MIM: 603566
UniProt ID: O75925
Chromosome Location: 15q
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; IFN-gamma pathway, organism-specific biosystem;
Function: DNA binding; SUMO ligase activity; androgen receptor binding; enzyme binding; ligase activity; metal ion binding; protein binding; transcription coactivator activity; transcription corepressor activity; ubiquitin protein ligase binding; zinc ion binding;

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