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Recombinant Human PEG10 293 Cell Lysate

Cat.No. : PEG10-3307HCL
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  • Gene Information
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Description : Antigen standard for paternally expressed 10 (PEG10), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PEG10 paternally expressed 10 [ Homo sapiens ]
Official Symbol : PEG10
Synonyms : PEG10; paternally expressed 10; retrotransposon-derived protein PEG10; HB 1; KIAA1051; Mar2; Mart2; MEF3L; RGAG3; MEF3 like 1; MEF3-like protein 1; ty3/Gypsy-like protein; paternally expressed gene 10 protein; retrotransposon gag domain containing 3; myelin expression factor 3-like protein 1; mammalian retrotransposon-derived protein 2; retrotransposon-derived gag-like polyprotein; embryonal carcinoma differentiation regulated; retrotransposon gag domain-containing protein 3; embryonal carcinoma differentiation-regulated protein; EDR; HB-1;
Gene ID : 23089
mRNA Refseq : NM_001040152
Protein Refseq : NP_001035242
MIM : 609810
UniProt ID : Q86TG7
Chromosome Location : 7q21
Pathway : Validated targets of C-MYC transcriptional activation, organism-specific biosystem;
Function : DNA binding; metal ion binding; protein binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the potential applications of PEG10 protein? 10/17/2023

PEG10 protein can be a candidate for drug development because of its role in regulating cell growth and differentiation. In addition, PEG10 protein can also be used to study the causes and mechanisms of liver disease.

How to detect the expression level of PEG10 protein? 05/22/2022

The expression level of PEG10 protein can be detected by immunohistochemistry, western blot or real-time quantitative PCR. These methods use specific antibodies to bind to the PEG10 protein, allowing qualitative and quantitative analysis of it.

What are the interactions between PEG10 and other proteins? 01/12/2022

This protein can interact with other proteins to regulate its function. For example, it may interact with proteins in transcription factors or other signal transduction pathways. By studying these interactions, we can further understand the mechanism of action of PEG10 protein.

Can the expression of PEG10 protein be regulated by gene knockout or knock-down technique? 02/03/2021

PEG10 protein can be regulated by using gene knockout or knock-down techniques. Gene knockout techniques reduce or eliminate the expression of a target gene by deleting it. Knock-down techniques inhibit the expression of target genes by using short hairpin RNA (shRNA) or small interfering RNA (siRNA). These techniques can be used to study the role of PEG10 protein in cell growth and differentiation.

What factors should be considered for drug development targeting PEG10 protein? 10/20/2020

Drug development targeting PEG10 requires consideration of a number of factors, including its specificity, safety, pharmacokinetic properties, and potential side effects. In addition, issues such as the dosage form, route of administration and design of clinical trials need to be considered. These factors will help ensure the success of drug development and the safety of patients.

Can the function of PEG10 protein be studied by means of structural biology? 07/03/2020

Structural biology approaches can help us understand the function of PEG10 protein. By analyzing the three-dimensional structure of PEG10 protein, we can understand the relationship between its shape and function. In addition, the conformation or function of PEG10 protein can also be changed by chemical biological methods, so as to study its mechanism of action.

Customer Reviews (3)

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Reviews
09/18/2022

    There will be no interaction with other drugs, and there will be no effects.

    09/09/2020

      It has good effect and stability in gene expression regulation.

      07/17/2020

        With PEG10, the efficiency of the experiment was significantly improved.

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