"PDE5A" Related Products

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Recombinant Human PDE5A 293 Cell Lysate

Cat.No.: PDE5A-3348HCL
Description: Antigen standard for phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC218974, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PDE5A phosphodiesterase 5A, cGMP-specific [ Homo sapiens ]
Official Symbol: PDE5A
Synonyms: PDE5A; phosphodiesterase 5A, cGMP-specific; cGMP-specific 3,5-cyclic phosphodiesterase; phosphodiesterase isozyme 5; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; cGMP-binding cGMP-specific 3,5-cyclic nucleotide phosphodiesterase; CN5A; PDE5; CGB-PDE;
Gene ID: 8654
mRNA Refseq: NM_001083
Protein Refseq: NP_001074
MIM: 603310
UniProt ID: O76074
Chromosome Location: 4q27
Pathway: Hemostasis, organism-specific biosystem; Nitric oxide stimulates guanylate cyclase, organism-specific biosystem; Platelet homeostasis, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; cGMP effects, organism-specific biosystem;
Function: 3,5-cyclic-GMP phosphodiesterase activity; 3,5-cyclic-nucleotide phosphodiesterase activity; cGMP binding; hydrolase activity; metal ion binding; nucleotide binding; zinc ion binding;

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