Active Recombinant Human PDE5A Protein, His-tagged

• Cat.No.: PDE5A-2567H
• Product Overview: Active Recombinant Human PDE5A Protein(Glu536~Gln860), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 536-860 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Bio-activity: Phosphodiesterase 5A (PDE5A) is a key enzyme that regulates cellular signaling by hydrolyzing cyclic guanosine monophosphate (cGMP), a second messenger involved in smooth muscle relaxation and vasodilation. It is best known as the therapeutic target for sildenafil (Viagra) in treating erectile dysfunction and pulmonary arterial hypertension, where its inhibition increases cGMP levels to enhance blood flow. Research also explores its roles in cardiac function and cancer. Meanwhile, PDE5A can form a functional complex with ALDH7A1, an aldehyde dehydrogenase, potentially linking cGMP signaling to cellular metabolism and stress response pathways.Thus a functional ELISA assay was conducted to detect the interaction of recombinant PDE5A and recombinant ALDH7A1. Briefly, PDE5A was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ALDH7A1-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PDE5A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant PDE5A and recombinant ALDH7A1 was shown, the EC50 for this effect is 0.005µg/mL.
• Molecular Mass: 41.3kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month, at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PDE5A phosphodiesterase 5A, cGMP-specific [ Homo sapiens ]
• Official Symbol: PDE5A
• Synonyms: PDE5A; phosphodiesterase 5A, cGMP-specific; cGMP-specific 3,5-cyclic phosphodiesterase; phosphodiesterase isozyme 5; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; cGMP-binding cGMP-specific 3,5-cyclic nucleotide phosphodiesterase; CN5A; PDE5; CGB-PDE
• Gene ID: 8654
• mRNA Refseq: NM_001083
• Protein Refseq: NP_001074
• MIM: 603310
• UniProt ID: O76074