"PAX5" Related Products

Recombinant Human PAX5 293 Cell Lysate

Cat.No.: PAX5-3416HCL
Description: Antigen standard for paired box 5 (PAX5) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PAX5 paired box 5 [ Homo sapiens ]
Official Symbol: PAX5
Synonyms: PAX5; paired box 5; paired box gene 5 (B cell lineage specific activator protein) , paired box gene 5 (B cell lineage specific activator); paired box protein Pax-5; B cell lineage specific activator; BSAP; paired box homeotic gene 5; transcription factor PAX 5; B cell specific activator protein; B-cell lineage specific activator; B-cell-specific transcription factor;
Gene ID: 5079
mRNA Refseq: NM_016734
Protein Refseq: NP_057953
MIM: 167414
UniProt ID: Q02548
Chromosome Location: 9p13.2
Pathway: C-MYB transcription factor network, organism-specific biosystem; Id Signaling Pathway, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;
Function: DNA binding; sequence-specific DNA binding transcription factor activity;
Products Types ◆ Recombinant Protein

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