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Recombinant Human NR1H4 293 Cell Lysate

Cat.No.: NR1H4-3719HCL
Description: Antigen standard for nuclear receptor subfamily 1, group H, member 4 (NR1H4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NR1H4 nuclear receptor subfamily 1, group H, member 4 [ Homo sapiens ]
Official Symbol: NR1H4
Synonyms: NR1H4; nuclear receptor subfamily 1, group H, member 4; bile acid receptor; FXR; HRR 1; HRR1; RIP14; farnesol receptor HRR-1; RXR-interacting protein 14; farnesoid X nuclear receptor; farnesoid X-activated receptor; retinoid X receptor-interacting protein 14; BAR; HRR-1; MGC163445;
Gene ID: 9971
mRNA Refseq: NM_001206977
Protein Refseq: NP_001193906
MIM: 603826
UniProt ID: Q96RI1
Chromosome Location: 12q23.1
Pathway: Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; Nuclear Receptor transcription pathway, organism-specific biosystem; Nuclear receptors in lipid metabolism and toxicity, organism-specific biosystem; RXR and RAR heterodimerization with other nuclear receptor, organism-specific biosystem;
Function: RNA polymerase II distal enhancer sequence-specific DNA binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; bile acid binding; bile acid binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; ligand-dependent nuclear receptor binding; metal ion binding; protein binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; steroid hormone receptor activity; thyroid hormone receptor activity; transcription coactivator activity; transcription corepressor activity; zinc ion binding;

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