Recombinant Human NLRC4 293 Cell Lysate

• Cat.No.: NLRC4-3805HCL

Specification

• Description: Antigen standard for NLR family, CARD domain containing 4 (NLRC4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NLRC4 NLR family, CARD domain containing 4 [ Homo sapiens ]
• Official Symbol: NLRC4
• Synonyms: NLRC4; NLR family, CARD domain containing 4; CARD12, caspase recruitment domain family, member 12; NLR family CARD domain-containing protein 4; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; ipaf; NOD like receptor C4; nucleotide binding oligomerization domain; leucine rich repeat and CARD domain containing 4; NOD-like receptor C4; ICE-protease activating factor; ice protease-activating factor; CARD, LRR, and NACHT-containing protein; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; IPAF; CARD12
• Gene ID: 58484
• mRNA Refseq: NM_001199138
• Protein Refseq: NP_001186067
• MIM: 606831
• UniProt ID: Q9NPP4
• Chromosome Location: 2p22-p21
• Pathway: Direct p53 effectors, organism-specific biosystem; Immune System, organism-specific biosystem; Inflammasomes, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Legionellosis, organism-specific biosystem; Legionellosis, conserved biosystem; NOD-like receptor signaling pathway, organism-specific biosystem
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein homodimerization activity