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Recombinant Human NEFM 293 Cell Lysate

Cat.No.: NEFM-3883HCL
Description: Antigen standard for neurofilament, medium polypeptide (NEFM), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NEFM neurofilament, medium polypeptide [ Homo sapiens ]
Official Symbol: NEFM
Synonyms: NEFM; neurofilament, medium polypeptide; NEF3, neurofilament, medium polypeptide 150kDa; neurofilament medium polypeptide; NF M; NFM; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa; NEF3; NF-M;
Gene ID: 4741
mRNA Refseq: NM_001105541
Protein Refseq: NP_001099011
MIM: 162250
UniProt ID: P07197
Chromosome Location: 8p21
Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem;
Function: protein binding; structural constituent of cytoskeleton;

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