"NDE1" Related Products


Recombinant Human NDE1 293 Cell Lysate

Cat.No. : NDE1-3938HCL
Description : Antigen standard for nudE nuclear distribution gene E homolog 1 (A. nidulans) (NDE1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : NDE1 nudE nuclear distribution E homolog 1 (A. nidulans) [ Homo sapiens ]
Official Symbol : NDE1
Synonyms : NDE1; nudE nuclear distribution E homolog 1 (A. nidulans); nudE nuclear distribution gene E homolog 1 (A. nidulans); nuclear distribution protein nudE homolog 1; FLJ20101; NUDE; nudE nuclear distribution gene E homolog 1; LIS1-interacting protein NUDE1, rat homolog; LIS4; NUDE1; HOM-TES-87;
Gene ID : 54820
mRNA Refseq : NM_001143979
Protein Refseq : NP_001137451
MIM : 609449
UniProt ID : Q9NXR1
Chromosome Location : 16p13.11
Pathway : Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; DNA Replication, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem;
Function : microtubule binding; protein binding;

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