"MYL3" Related Products

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Recombinant Human MYL3 293 Cell Lysate

Cat.No.: MYL3-4027HCL
Description: Antigen standard for myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MYL3 myosin, light chain 3, alkali; ventricular, skeletal, slow [ Homo sapiens ]
Official Symbol: MYL3
Synonyms: MYL3; myosin, light chain 3, alkali; ventricular, skeletal, slow; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow; myosin light chain 3; CMH8; MLC1SB; MLC1V; VLC1; CMLC1; cardiac myosin light chain 1; ventricular/slow twitch myosin alkali light chain; myosin light chain 1, slow-twitch muscle B/ventricular isoform; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow;
Gene ID: 4634
mRNA Refseq: NM_000258
Protein Refseq: NP_000249
MIM: 160790
UniProt ID: P08590
Chromosome Location: 3p
Pathway: Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), conserved biosystem; Muscle contraction, organism-specific biosystem;
Function: actin monomer binding; calcium ion binding; motor activity; myosin II heavy chain binding; structural constituent of muscle;

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