"MTF1" Related Products

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Recombinant Human MTF1 293 Cell Lysate

Cat.No.: MTF1-4085HCL
Description: Antigen standard for metal-regulatory transcription factor 1 (MTF1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MTF1 metal-regulatory transcription factor 1 [ Homo sapiens ]
Official Symbol: MTF1
Synonyms: MTF1; metal-regulatory transcription factor 1; metal regulatory transcription factor 1; zinc regulatory factor; transcription factor MTF-1; MRE-binding transcription factor; MRE-binding transcription factor-1; metal-responsive transcription factor 1; ZRF; MTF-1; MGC23036;
Gene ID: 4520
mRNA Refseq: NM_005955
Protein Refseq: NP_005946
MIM: 600172
UniProt ID: Q14872
Chromosome Location: 1p33
Function: DNA binding; RNA polymerase II core promoter sequence-specific DNA binding; core promoter binding; histone acetyltransferase binding; metal ion binding; protein binding; sequence-specific DNA binding transcription factor activity; transcription coactivator activity; zinc ion binding;

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