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Recombinant Human MRC1 293 Cell Lysate

Cat.No.: MRC1-4212HCL
Description: Antigen standard for mannose receptor, C type 1 (MRC1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: MRC1 mannose receptor, C type 1 [ Homo sapiens ]
Official Symbol: MRC1
Synonyms: MRC1; mannose receptor, C type 1; mannose receptor, C type 1 like 1 , MRC1L1; macrophage mannose receptor 1; bA541I19.1; CD206; CLEC13D; CLEC13DL; mannose receptor, C type 1-like 1; C-type lectin domain family 13 member D; macrophage mannose receptor 1-like protein 1; MMR; MRC1L1; MGC181940;
Gene ID: 4360
mRNA Refseq: NM_002438
Protein Refseq: NP_002429
MIM: 153618
UniProt ID: P22897
Chromosome Location: 10p13
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; Cross-presentation of soluble exogenous antigens (endosomes), organism-specific biosystem; Immune System, organism-specific biosystem; Phagosome, organism-specific biosystem;
Function: binding; mannose binding; receptor activity; sugar binding;

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