Recombinant Human MECOM 293 Cell Lysate

• Cat.No.: MECOM-4396HCL

Specification

• Description: Antigen standard for MDS1 and EVI1 complex locus (MECOM), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MECOM MDS1 and EVI1 complex locus [ Homo sapiens ]
• Official Symbol: MECOM
• Synonyms: MECOM; MDS1 and EVI1 complex locus; ecotropic viral integration site 1 , EVI1, MDS1, myelodysplasia syndrome 1; MDS1 and EVI1 complex locus protein EVI1; MDS1 EVI1; PRDM3; oncogene EVI1; zinc finger protein Evi1; AML1-EVI-1 fusion protein; MDS1 and EVI1 complex locus protein MDS1; myelodysplasia syndrome-associated protein 1; ecotropic virus integration site 1 protein homolog; EVI1; MDS1; MDS1-EVI1; AML1-EVI-1; MGC97004; MGC163392
• Gene ID: 2122
• mRNA Refseq: NM_001105077
• Protein Refseq: NP_001098547
• MIM: 165215
• UniProt ID: Q03112
• Chromosome Location: 3q26
• Pathway: Chronic myeloid leukemia, organism-specific biosystem; Chronic myeloid leukemia, conserved biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; Pathways in cancer, organism-specific biosystem
• Function: DNA binding; DNA binding; metal ion binding; protein binding; protein homodimerization activity; NOT protein homodimerization activity; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; zinc ion binding