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Recombinant Human KLF4 293 Cell Lysate

Cat.No.: KLF4-4927HCL
Description: Antigen standard for Kruppel-like factor 4 (gut) (KLF4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: KLF4 Kruppel-like factor 4 (gut) [ Homo sapiens ]
Official Symbol: KLF4
Synonyms: KLF4; Kruppel-like factor 4 (gut); Krueppel-like factor 4; EZF; GKLF; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein;
Gene ID: 9314
mRNA Refseq: NM_004235
Protein Refseq: NP_004226
MIM: 602253
UniProt ID: O43474
Chromosome Location: 9q31
Pathway: Developmental Biology, organism-specific biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Synthesis, Secretion, and Deacylation of Ghrelin, organism-specific biosystem; Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem;
Function: DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding; RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; core promoter proximal region sequence-specific DNA binding; double-stranded DNA binding; metal ion binding; phosphatidylinositol 3-kinase regulator activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; sequence-specific DNA binding transcription factor recruiting transcription factor activity; transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding;

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