"KLF11" Related Products

Recombinant Human KLF11 293 Cell Lysate

Cat.No.: KLF11-4932HCL
Description: Antigen standard for Kruppel-like factor 11 (KLF11) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: KLF11 Kruppel-like factor 11 [ Homo sapiens ]
Official Symbol: KLF11
Synonyms: KLF11; Kruppel-like factor 11; TGFB inducible early growth response 2 , TIEG2; Krueppel-like factor 11; MODY7; Tieg3; TIEG-2; TGFB-inducible early growth response protein 2; transforming growth factor-beta-inducible early growth response protein 2; FKLF; FKLF1; TIEG2;
Gene ID: 8462
mRNA Refseq: NM_001177716
Protein Refseq: NP_001171187
MIM: 603301
UniProt ID: O14901
Chromosome Location: 2p25
Pathway: EGFR1 Signaling Pathway, organism-specific biosystem;
Function: DNA binding; metal ion binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding; zinc ion binding;

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