"ITIH2" Related Products

Recombinant Human ITIH2 293 Cell Lysate

Cat.No.: ITIH2-5119HCL
Description: Antigen standard for inter-alpha (globulin) inhibitor H2 (ITIH2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ITIH2 inter-alpha-trypsin inhibitor heavy chain 2 [ Homo sapiens ]
Official Symbol: ITIH2
Synonyms: ITIH2; inter-alpha-trypsin inhibitor heavy chain 2; inter alpha (globulin) inhibitor, H2 polypeptide; inter-alpha-trypsin inhibitor heavy chain H2; H2P; ITI-HC2; ITI heavy chain H2; inter-alpha (globulin) inhibitor H2; inter-alpha-inhibitor heavy chain 2; serum-derived hyaluronan-associated protein; inter-alpha (globulin) inhibitor, H2 polypeptide; inter-alpha-trypsin inhibitor complex component II; SHAP;
Gene ID: 3698
mRNA Refseq: NM_002216
Protein Refseq: NP_002207
MIM: 146640
UniProt ID: P19823
Chromosome Location: 10p14
Function: endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity;

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