"ISL1" Related Products


Recombinant Human ISL1 293 Cell Lysate

Cat.No.: ISL1-5149HCL
Description: Antigen standard for ISL LIM homeobox 1 (ISL1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ISL1 ISL LIM homeobox 1 [ Homo sapiens ]
Official Symbol: ISL1
Synonyms: ISL1; ISL LIM homeobox 1; ISL1 transcription factor, LIM/homeodomain, (islet 1); insulin gene enhancer protein ISL-1; Isl 1; ISLET1; islet-1; ISL1 transcription factor, LIM/homeodomain; Isl-1;
Gene ID: 3670
mRNA Refseq: NM_002202
Protein Refseq: NP_002193
MIM: 600366
UniProt ID: P61371
Chromosome Location: 5q11.2
Pathway: Heart Development, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Synthesis, Secretion, and Inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP), organism-specific biosystem;
Function: RNA polymerase II activating transcription factor binding; RNA polymerase II transcription coactivator activity; bHLH transcription factor binding; chromatin binding; enhancer sequence-specific DNA binding; enhancer sequence-specific DNA binding; estrogen receptor binding; ligand-dependent nuclear receptor binding; metal ion binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; zinc ion binding;

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