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Recombinant Human INSIG1 293 Cell Lysate

Cat.No. : INSIG1-5193HCL
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  • Gene Information
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Description : Antigen standard for insulin induced gene 1 (INSIG1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : INSIG1 insulin induced gene 1 [ Homo sapiens ]
Official Symbol : INSIG1
Synonyms : INSIG1; insulin induced gene 1; insulin-induced gene 1 protein; CL 6; INSIG 1 membrane protein; MGC1405; INSIG-1 membrane protein; CL6; CL-6;
Gene ID : 3638
mRNA Refseq : NM_005542
Protein Refseq : NP_005533
MIM : 602055
UniProt ID : O15503
Chromosome Location : 7q36
Function : protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does INSIG1 contribute to cellular lipid droplet formation? 04/14/2022

INSIG1 contributes to cellular lipid droplet formation by influencing lipid homeostasis and storage in response to sterol levels.

Is INSIG1 involved in the regulation of triglyceride metabolism? 01/08/2022

While INSIG1 primarily regulates cholesterol synthesis, its indirect influence on triglyceride metabolism is observed through modulation of SREBP-regulated pathways.

How does INSIG1 impact the degradation of HMG-CoA reductase? 02/09/2020

INSIG1 promotes the degradation of HMG-CoA reductase, a key enzyme in cholesterol synthesis, through ubiquitination and proteasomal degradation.

Are there any known inhibitors or activators of INSIG1? 12/07/2018

There are no widely recognized small-molecule inhibitors or activators of INSIG1 identified to date.

What is the significance of INSIG1 in the feedback regulation of cholesterol synthesis? 03/28/2018

INSIG1 is crucial in the feedback regulation of cholesterol synthesis, preventing excess cholesterol production by inhibiting the activation of SREBP proteins.

Does INSIG1 expression respond to changes in cellular sterol levels? 08/08/2017

Yes, the expression of INSIG1 is responsive to changes in cellular sterol levels, serving as a key component of the sterol-sensing mechanism.

What is the role of INSIG1 in the unfolded protein response (UPR)? 02/26/2017

INSIG1 is involved in the unfolded protein response (UPR), a cellular stress response pathway associated with endoplasmic reticulum (ER) stress.

Customer Reviews (3)

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Reviews
07/19/2021

    We found the product's safety and biocompatibility reassuring, especially when handling sensitive samples.

    07/23/2018

      Precise immunoprecipitation results consistently reflected the product's quality.

      07/20/2017

        The product's innovative characteristics enabled us to delve into previously uncharted cellular mechanisms.

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